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Federal Drug Testing Rule Will Require ‘Directly Observed’ Urine Collection from Truck Drivers

Jul 24, 2026 | Pre-employment Drug Testing, Transportation Industry Hiring

The U.S. Department of Transportation has finalized a drug-testing rule requiring directly observed urine collection in certain cases involving safety-sensitive workers, including truck drivers. The rule applies when oral fluid testing is unavailable or impractical due to limited lab capacity and is intended to prevent tampering or substitution. Officials say it strengthens the integrity of federal drug-testing programs while oral fluid testing infrastructure continues to develop. The change is part of broader updates modernizing DOT testing procedures.

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